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lr clonase ii enzyme mix  (Thermo Fisher)


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    Structured Review

    Thermo Fisher lr clonase ii enzyme mix
    Lr Clonase Ii Enzyme Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lr+clonase+ii+enzyme/gateway+lr+clonase+ii+enzyme+mix/pm40650235-262-16-21
    Average 90 stars, based on 1 article reviews
    lr clonase ii enzyme mix - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Expressing:

    Article Title: dFLASH; dual FLuorescent transcription factor activity sensor for histone integrated live-cell reporting and high-content screening.
    Article Snippet: The Lentiviral backbone expression construct pLV-TET2BLASTGtwyA was then used to insert expression constructs cloned into pENTR1a by LR Clonase II enzyme recombination (Cat#11791020, Thermo). .. The Lentiviral backbone expression construct pLV-TET2BLASTGtwyA was then used to insert expression constructs cloned into pENTR1a by LR Clonase II enzyme recombination (Cat#11791020, Thermo). ..

    Construct:

    Article Title: dFLASH; dual FLuorescent transcription factor activity sensor for histone integrated live-cell reporting and high-content screening.
    Article Snippet: The Lentiviral backbone expression construct pLV-TET2BLASTGtwyA was then used to insert expression constructs cloned into pENTR1a by LR Clonase II enzyme recombination (Cat#11791020, Thermo). .. The Lentiviral backbone expression construct pLV-TET2BLASTGtwyA was then used to insert expression constructs cloned into pENTR1a by LR Clonase II enzyme recombination (Cat#11791020, Thermo). ..

    Clone Assay:

    Article Title: dFLASH; dual FLuorescent transcription factor activity sensor for histone integrated live-cell reporting and high-content screening.
    Article Snippet: The Lentiviral backbone expression construct pLV-TET2BLASTGtwyA was then used to insert expression constructs cloned into pENTR1a by LR Clonase II enzyme recombination (Cat#11791020, Thermo). .. The Lentiviral backbone expression construct pLV-TET2BLASTGtwyA was then used to insert expression constructs cloned into pENTR1a by LR Clonase II enzyme recombination (Cat#11791020, Thermo). ..

    Article Title: The HPV101 E7 protein shares host cellular targets and biological activities with high-risk HPV16 E7.
    Article Snippet: To generate the pLenti-CMV-Blast-DEST plasmids, HPV101 E7 117 was PCR amplified using the primers listed in Table S1 and cloned into pENTR-D-118 TOPO (Invitrogen) with the pENTR-D-TOPO cloning kit (Invitrogen, K240020SP). .. 119 Positive clones were selected, and the HPV101 E7 was transferred to pLenti-CMV-120 Blast-Dest [21] using the LR Clonase II enzyme (Invitrogen, 11791020). .. All expression 121 plasmids were verified by whole-plasmid sequencing by Plasmidsaurus using Oxford 122 Jo urn l P repro of 5 Nanopore Technology with custom analysis and annotation.

    Article Title: Characterization of FchAGL9 and FchSHP, two MADS-boxes related to softening of Fragaria chiloensis fruit.
    Article Snippet: LUC/REN ratio activity was measured using the Tecan Microplate Luminometer as described by Espley et al. (2009), and reported as mean of four technical replicates ± SD. .. For over-expression assays, FchAGL9 and FchSHP CDS were cloned M. Zamorano-Curaqueo et al. Plant Physiology and Biochemistry 215 (2024) 108985 from the pENTR/SD vector into the pK7WG2 vector using the LR Clonase-II enzyme (Invitrogen). ..

    Plasmid Preparation:

    Article Title: Characterization of FchAGL9 and FchSHP, two MADS-boxes related to softening of Fragaria chiloensis fruit.
    Article Snippet: .. CDS of FchAGL9 and FchSHPwithout stop codon were independently recombined into pK7FWG2 vector, which contains the green fluorescent protein (GFP) reporter gene under the control of Cauliflower Mosaic Virus (CaMV) 35S promoter, using LR Clonase-II enzyme (Invitrogen). ..

    Article Title: De Novo Variants in MRTFB have gain of function activity in Drosophila and are associated with a novel neurodevelopmental phenotype with dysmorphic features
    Article Snippet: Variants were generated in the pDONR vector via site-directed mutagenesis utilizing NEBaseChanger with the Q5 mutagenesis kit (NEB) and verified by sequencing. .. LR clonase II (ThermoFisher) enzyme was then used to shuttle sequence verified reference and variant ORFs into the p.UASg-HA.attB destination vector. ..

    Control:

    Article Title: Characterization of FchAGL9 and FchSHP, two MADS-boxes related to softening of Fragaria chiloensis fruit.
    Article Snippet: .. CDS of FchAGL9 and FchSHPwithout stop codon were independently recombined into pK7FWG2 vector, which contains the green fluorescent protein (GFP) reporter gene under the control of Cauliflower Mosaic Virus (CaMV) 35S promoter, using LR Clonase-II enzyme (Invitrogen). ..

    Virus:

    Article Title: Characterization of FchAGL9 and FchSHP, two MADS-boxes related to softening of Fragaria chiloensis fruit.
    Article Snippet: .. CDS of FchAGL9 and FchSHPwithout stop codon were independently recombined into pK7FWG2 vector, which contains the green fluorescent protein (GFP) reporter gene under the control of Cauliflower Mosaic Virus (CaMV) 35S promoter, using LR Clonase-II enzyme (Invitrogen). ..

    Transformation Assay:

    Article Title: Sensitivity to Cuticular Hydrocarbons Across the Odorant Receptor Family in the Indian Jumping Ant
    Article Snippet: .. HsOrs in entry vectors from both approaches were transformed, purified, and sequenced, and then subsequently subcloned into pUASg.attB (gift from Konrad Basler, University of Zurich) with LR Clonase II enzyme (Invitrogen, ThermoFisher Scientific, Waltham, MA, USA). .. Resulting pUASg.attB-HsOr plasmids were then injected into Drosophila melanogaster embryos from the attp40w line with a genetically encoded phiC31 integrase (Rainbow Transgenic Flies Inc., Camarillo, CA, USA).

    Purification:

    Article Title: Sensitivity to Cuticular Hydrocarbons Across the Odorant Receptor Family in the Indian Jumping Ant
    Article Snippet: .. HsOrs in entry vectors from both approaches were transformed, purified, and sequenced, and then subsequently subcloned into pUASg.attB (gift from Konrad Basler, University of Zurich) with LR Clonase II enzyme (Invitrogen, ThermoFisher Scientific, Waltham, MA, USA). .. Resulting pUASg.attB-HsOr plasmids were then injected into Drosophila melanogaster embryos from the attp40w line with a genetically encoded phiC31 integrase (Rainbow Transgenic Flies Inc., Camarillo, CA, USA).

    other:

    Article Title: The HPV101 E7 protein shares host cellular targets and biological activities with high-risk HPV16 E7
    Article Snippet: To generate the pLenti-CMV-Blast-DEST plasmids, HPV101 E7 was PCR amplified using the primers listed in and cloned into pENTR-D-TOPO (Invitrogen) with the pENTR-D-TOPO cloning kit (Invitrogen, K240020SP).

    Sequencing:

    Article Title: De Novo Variants in MRTFB have gain of function activity in Drosophila and are associated with a novel neurodevelopmental phenotype with dysmorphic features
    Article Snippet: Variants were generated in the pDONR vector via site-directed mutagenesis utilizing NEBaseChanger with the Q5 mutagenesis kit (NEB) and verified by sequencing. .. LR clonase II (ThermoFisher) enzyme was then used to shuttle sequence verified reference and variant ORFs into the p.UASg-HA.attB destination vector. ..

    Variant Assay:

    Article Title: De Novo Variants in MRTFB have gain of function activity in Drosophila and are associated with a novel neurodevelopmental phenotype with dysmorphic features
    Article Snippet: Variants were generated in the pDONR vector via site-directed mutagenesis utilizing NEBaseChanger with the Q5 mutagenesis kit (NEB) and verified by sequencing. .. LR clonase II (ThermoFisher) enzyme was then used to shuttle sequence verified reference and variant ORFs into the p.UASg-HA.attB destination vector. ..



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